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Molecular characterization of Carthamus tinctorius and C. oxyacanthus germplasm using sequence related amplified polymorphism (SRAP) markers

Plant Omics5.2 (Mar 2012): 136-142.

Talebi, Majid; Mokhtari, Niloofar; Rahimmalek, Mehdi; Sahhafi, Seyyed Rasoul.


Genetic diversity of 42 Carthamus oxyacanthus and seven C. tinctorius genotypes from five distinct geographical regions of Iran was evaluated using sequence related amplified polymorphism (SRAP) markers. Twelve SRAP primer combinations (PCs) amplified 293 fragments, of which 224 were polymorphic. The number of polymorphic fragments detecting per PC ranged from 13 to 24 bands with an average of 18.66. Average PIC value was 0.339 over all PCs. Cluster analysis using UPGMA method and Jaccard's similarity coefficient (r = 0.85) grouped the genotypes into five main clusters according to species and regions. Sum of the first three PCOs could represent most of (75.2%) the total variation in the original dimensions and confirm the results of cluster analysis. The obtained population genetic indexes revealed considerable diversity and heterozygous nature of C. oxyacanthus in comparison to C. tinctorius. The AMOVA results also showed significant differences in the genetic diversity among the species (Fst = 0.277; P < 0.0001) and regions (Fst = 0.185; P < 0.0001). The results revealed the existence of wide genetic base of safflower germplasm in Iran and the effectiveness of SRAP markers for studying genetic diversity and relationships among and within species and regions.

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