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Genetic relationships among Achillea tenuifolia accessions using molecular and morphological markers

 Plant Omics5.2 (Mar 2012): 128-135.

Rahimmalek, M.

ISSR and morphological markers were used to detect genetic diversity in several genotypes of Achillea tenuifolia from different geographical regions of Iran. Fifteen primers revealed 247 polymorphic bands, out of which 214 (86.78%) were polymorphic. The dendrogram was constructed using SM coefficient and UPGMA method. The generated dendrogram revealed three groups. The accessions originated from central regions of the country separated from others in group 3. The principle coordinate analysis (PCoA) confirmed the results of clustering (>90%). For morphological traits, North-western (NW) accessions had the highest values of leaf length, leaf width, leaf area, essential oil yield and the latest flowering time, while the Northern one (AtN76) had the highest flower diameter and number of florets in main inflorescence. Results showed the relatively broad genetic base of in most of the accessions evaluated in this study. The lowest and the highest gene diversity were obtained in North-western (AtNW) group (0.18) and Northern (AtN) accession (0.28) respectively. High genetic variation of A. tenuifolia might be attributed to its reproductive propagation and seed dispersal. So, conservation strategies should be provided to maintain such diversity aiming to improve future breeding programs.
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